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Table 2 Information on the MMR protein antibodies used

From: Heterogenous mismatch-repair status in colorectal cancer

MMR protein

Lab

Supplier

Clone

Dilution

Immunogen/epitope

MLH1

L

BD Pharmingen

G168-15

1:100

Full-length

 

C

DAKO

ES05

RTU

Recombinant protein, 210 aa

 

L

DAKO

ES05

1:100

Recombinant protein, 210 aa

PMS2

L

BD Pharmingen

A16-4

1:300

aa 431–862, C-terminal

 

H

Ventana (Cell Marque)

EPR3947

RTU

100 aa, C-terminal

 

C

Epitomics

EPR3947

1:50

100 aa, C-terminal

MSH2

L

Calbiochem

FE11

1:100

C-terminal

 

H

Ventana (Cell Marque)

G219-1129

RTU

Full-length

 

C

Novocastra

25D12

1:50

Full-length

MSH6

C

Epitomics

EP49

1:100

Synthetic peptide, N-terminal

 

L

Epitomics

EP3945

1:100

Synthetic peptide, N-terminal

 

L

BD Transduction Lab.

44

1:500

Synthectic peptide aa 225-333

  1. MMR: mismatch repair; L: Lund; H Helsingborg; C: Copenhagen; RTU: ready to use.