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Table 1 PTEN primers used for single-strand conformation polymorphism (SSCP) analysis

From: Occipital anaplastic oligodendroglioma with multiple organ metastases after a short clinical course: a case report and literature review

Exon Primer sequences Annealing temp. (°C) Amplicon (bp)
1 F1. 5’-TCCTCCTTTTTCTTCAGCCAC-3’ 56 147
  R1. 5’-GAAAGGTAAAGAGGAGCAGCC-3’   
2 F2. 5’-TGCATATTTCAGATATTTCTTTCCTT-3’ 57 155
  R2. 5’-TTTGAAATAGAAAATCAAAGCATTC-3’   
3 F3. 5’-TGTTAATGGTGGCTTTTTG-3’ 56 114
  R3. 5’-GCAAGCATACAAATAAGAAAAC-3’   
4 F4. 5’-TTCCTAAGTGCAAAAGATAAC-3’ 56 147
  R4. 5’-TACAGTCTATCGGGTTTAAGT-3’   
5 F5. 5’-TTTTTTTTTCTTATTCTGAGGTTATC-3’ 51 312
  R5. 5’-GAAGAGGAAAGGAAAAACATC-3’   
6 F6. 5’-AGTGAAATAACTATAATGGAACA-3’ 54 231
  R6. 5’-GAAGGATGAGAATTTCAAGC-3’   
7 F7. 5’-ATCGTTTTTGACAGTTTG-3’ 55 262
  R7. 5’-TCCCAATGAAAGTAAAGTAGA-3’   
8 F8. 5’-AGGTGACAGATTTTCTTTTTTA-3’ 52 394
  R8. 5’-TAGCTGTACTCCTAGAATTA-3’   
9 F9. 5’-CTTTCTCTAGGTFAAGCTGTACTT-3’ 55 231
  R9. 5’-TTCATGGTGTTTTATCCCTCTTGA-3’