Table 1 PTEN primers used for single-strand conformation polymorphism (SSCP) analysis
Exon | Primer sequences | Annealing temp. (°C) | Amplicon (bp) |
|---|---|---|---|
1 | F1. 5’-TCCTCCTTTTTCTTCAGCCAC-3’ | 56 | 147 |
R1. 5’-GAAAGGTAAAGAGGAGCAGCC-3’ | |||
2 | F2. 5’-TGCATATTTCAGATATTTCTTTCCTT-3’ | 57 | 155 |
R2. 5’-TTTGAAATAGAAAATCAAAGCATTC-3’ | |||
3 | F3. 5’-TGTTAATGGTGGCTTTTTG-3’ | 56 | 114 |
R3. 5’-GCAAGCATACAAATAAGAAAAC-3’ | |||
4 | F4. 5’-TTCCTAAGTGCAAAAGATAAC-3’ | 56 | 147 |
R4. 5’-TACAGTCTATCGGGTTTAAGT-3’ | |||
5 | F5. 5’-TTTTTTTTTCTTATTCTGAGGTTATC-3’ | 51 | 312 |
R5. 5’-GAAGAGGAAAGGAAAAACATC-3’ | |||
6 | F6. 5’-AGTGAAATAACTATAATGGAACA-3’ | 54 | 231 |
R6. 5’-GAAGGATGAGAATTTCAAGC-3’ | |||
7 | F7. 5’-ATCGTTTTTGACAGTTTG-3’ | 55 | 262 |
R7. 5’-TCCCAATGAAAGTAAAGTAGA-3’ | |||
8 | F8. 5’-AGGTGACAGATTTTCTTTTTTA-3’ | 52 | 394 |
R8. 5’-TAGCTGTACTCCTAGAATTA-3’ | |||
9 | F9. 5’-CTTTCTCTAGGTFAAGCTGTACTT-3’ | 55 | 231 |
R9. 5’-TTCATGGTGTTTTATCCCTCTTGA-3’ |