Expression analysis of ERβ, ERα, PR and Her-2 in breast cancer tissue. Immunohistochemistry was performed to detect ERβ, ERα, PR and Her-2 expression. Representative images were shown. Cells with brown granules were positive cells. Scale bar, 100 μm. (A) ERβ negative tissue (ERβ (−)). (B) ERβ weak positive expression tissue (ERβ (+), cancer cells with a positive rate of < 10%). (C) ERβ positive expression tissue (ERβ (++), cancer cells with a positive rate between 10% and 50%). (D) ERβ high expression tissue (ERβ (+++), cancer cells with a positive rate of > 50%). (E) ERα negative tissue. (F) ERα positive tissue. (G) HR negative tissue. (H) HR positive tissue. (I) Her-2 negative tissue. (J) Her-2 positive tissue.