Table 2 VENTANA IHC and qRT-PCR analysis of all weakly positive and discordant cases detected by CST ALK (D5F3)
From: Combination of conventional immunohistochemistry and qRT-PCR to detect ALK rearrangement
Sample ID | FISH | IHC (CST) | IHC (VENTANA) | qRT-PCR |
|---|---|---|---|---|
Variant type | ||||
9 | Positive | 1+ | Positive | EML4-ALK variant 1/2/3a/3b |
37 | Positive | 1+ | Positive | EML4-ALK variant 1/2/3a/3b |
67 | Positive | 1+ | Positive | EML4-ALK variant 1/2/3a/3b |
94 | Positive | 1+ | Positive | EML4-ALK variant 1/2/3a/3b |
98 | Positive | 1+ | Positive | EML4-ALK variant 1/2/3a/3b |
28 | Negative | 2+ | Positive | EML4-ALK variant 1/2/3a/3b |
171 | Negative | 1+ | Positive | EML4-ALK variant 1/2/3a/3b |
203 | Negative | 1+ | Positive | EML4-ALK variant 1/2/3a/3b |
21 | Negative | 1+ | Negative | EML4-ALK variant 1/2/3a/3b |
36 | Negative | 1+ | Negative | EML4-ALK variant 1/2/3a/3b |
41 | Negative | 1+ | Negative | Negative |
39 | Negative | 1+ | Negative | Negative |
74 | Negative | 1+ | Negative | Negative |