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Table 4 The four classes of reagents used in glycocyto- and histochemical analysis

From: Introduction to glycopathology: the concept, the tools and the perspectives

Experimental aim

Type of reagent

Example

Detect certain aspects of glycosylation

Plant lectin/carbohydrate-specific antibody

Monitoring the presence of β1,6-branching in N-glycans or of sialylated Lewis epitopes

Detect accessible sites binding a distinct carbohydrate epitope

Neoglycoconjugate

Detecting binding sites for sialylated Lewis epitopes in colon cancer

Detect distinct lectins in situ

Antibody specific for endogenous lectin

Performing immunohistochemical galectin fingerprinting in colon cancer (with prognostic relevance for galectins)

Detect accessible ligands (glycan/peptide) for an endogenous lectin

Tissue lectin

Delineating prognostic relevance for galectin-3 binding in head and neck cancer sections

  1. From [73], with permission.