From: Introduction to glycopathology: the concept, the tools and the perspectives
Experimental aim | Type of reagent | Example |
---|---|---|
Detect certain aspects of glycosylation | Plant lectin/carbohydrate-specific antibody | Monitoring the presence of β1,6-branching in N-glycans or of sialylated Lewis epitopes |
Detect accessible sites binding a distinct carbohydrate epitope | Neoglycoconjugate | Detecting binding sites for sialylated Lewis epitopes in colon cancer |
Detect distinct lectins in situ | Antibody specific for endogenous lectin | Performing immunohistochemical galectin fingerprinting in colon cancer (with prognostic relevance for galectins) |
Detect accessible ligands (glycan/peptide) for an endogenous lectin | Tissue lectin | Delineating prognostic relevance for galectin-3 binding in head and neck cancer sections |