Table 2 Q-MSP primer and TaqMan probe sequences used in this study
Genes | Forward primer sequences (5′ → 3′) | Probe sequence | Reverse primer sequence (5′ → 3′) |
|---|---|---|---|
CALCA | GTTTTGGAAGTATGAGGGTGACG | 6FAM-ATTCCGCCAATACACAACAACCAATAAACG-TAMRA | TTCCCGCCGCTATAAATCG |
DAPK1 | GGATAGTCGGATCGAGTTAACGTC | 6FAM-TTCGGTAATTCGTAGCGGTAGGGTTTGG-TAMRA | CCCTCCCAAACGCCGA |
TIMP3 | GCGTCGGAGGTTAAGGTTGTT | 6FAM-AACTCGCTCGCCCGCCGAA-TAMRA | CTCTCCAAAATTACCGTACGCG |
RAR-beta | GGGATTAGAATTTTTTATGCGAGTTGT | 6FAM-TGTCGAGAACGCGAGCGATTCG-TAMRA | TACCCCGACGATACCCAAAC |
RASSF1A | GCGTTGAAGTCGGGGTTC | 6FAM-ACAAACGCGAACCGAACGAAACCA-TAMRA | CCCGTACTTCGCTAACTTTAAACG |
β-actin | TGGTGATGGAGGAGGTTTAGTAAGT | 6FAM-ACCACCACCCAACACACAATAACAAACACA-TAMR | AAACCAATAAAACCTACTCCTCCCTTAA |