Table 4 Diagnostic values of combined detection of BRAF mutation and various DNA methylation markers on FNAB specimens for PTC detection [percent (PTCs with BRAF mutation or methylation-positive/total PTCs, sensitivity; benign nodules with BRAF mutation or methylation-negative/total benign nodules, specificity; PTCs with BRAF mutation or methylation-positive/total positive cases, PPV; benign nodules with BRAF mutation or methylation-negative/total negative nodules, NPV)]
| Â | Cut-off values | Training group (n =79) | ||||
|---|---|---|---|---|---|---|
Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Accuracy (%) | ||
BRAF V600E | N/A | 71.43 (30/42) | 100 (37/37) | 100 (30/30) | 75.51 (37/49) | 71.43 |
CALCA + BRAF V600E | 8.022 | 71.43 (30/42) | 100 (37/37) | 100 (30/30) | 75.51 (37/49) | 71.43 |
DAPK1 + BRAF V600E | 0.64 | 78.57 (33/42) | 100 (37/37) | 100 (33/33) | 80.43 (37/46) | 78.57 |
TIMP3 + BRAF V600E | 6.46 | 73.81 (31/42) | 100 (37/37) | 100 (31/31) | 77.08 (37/48) | 73.81 |
RAR-beta + BRAF V600E | 11.21 | 73.81 (31/42) | 100 (37/37) | 100 (31/31) | 77.08 (37/48) | 73.81 |
RASSF1A + BRAF V600E | 0.003 | 76.19 (32/42) | 100 (37/37) | 100 (32/32) | 78.72 (37/47) | 76.19 |
All five genes + BRAFV600E | N/A | 85.71 (36/42) | 100 (37/37) | 100 (36/36) | 86.05 (37/43) | 85.71 |
| Â | Cut-off values | Test group (n =38) | ||||
Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Accuracy (%) | ||
BRAF V600E | N/A | 70.00 (14/20) | 100 (18/18) | 100 (14/14) | 75.00 (18/24) | 70.00 |
CALCA + BRAF V600E | 8.022 | 75.00 (15/20) | 100 (18/18) | 100 (15/15) | 78.26 (18/23) | 75.00 |
DAPK1 + BRAF V600E | 0.64 | 80.00 (16/20) | 94.44 (17/18) | 94.12 (16/17) | 80.95 (17/21) | 74.44 |
TIMP3 + BRAF V600E | 6.46 | 75.00 (15/20) | 100 (18/18) | 100 (15/15) | 78.26 (18/23) | 75.00 |
RAR-beta + BRAF V600E | 11.21 | 75.00 (15/20) | 100 (18/18) | 100 (15/15) | 78.26 (18/23) | 75.00 |
RASSF1A + BRAF V600E | 0.003 | 75.00 (15/20) | 100 (18/18) | 100 (15/15) | 78.26 (18/23) | 75.00 |
All five genes + BRAFV600E | N/A | 85.00 (17/20) | 94.44 (17/18) | 94.44 (17/18) | 85.00 (17/20) | 79.44 |