RETRACTED ARTICLE: Detection of Helicobacter spp. DNA in the colonic biopsies of stray dogs: molecular and histopathological investigations

Table 1 Age, gender, clinical signs, inflammatory and morphological changes and colonization status of Helicobacter spirochetes

Dog no.		1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
Gender^a		M	F	M	M	M	F	F	M	F	F	F	F	M	M	M
Age^b		1	3	1	2	3	1	2	3	3	1	3	1	2	1	3
Clinical signs^c		MD, W,A	H	MD, W,A	H	MD, W,A	MD, W,A	H	H	H	MD, W,A	H	H	MD, W,A	H	H
Persistence of Helicobacter spirochetes	Mucosal surface	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
Persistence of Helicobacter spirochetes	Crypt	+	+	+	-	-	+	-	-	-	+	-	+	+	-	-
Helicobacter spirochete colonization status^d		+3	+2	+2	+1	+1	+2	+1	+1	+1	+3	+1	+2	+1	+2	+1
Inflammatory cell Infiltration^e	LP	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
	N	+	+	+	-	-	+	-	-	-	+	-	+	+	-	-
	E	+	-	+	-	-	-	-	-	-	+	-	-	-	-	-
	M	-	+	+	-	-	+	+	-	+	+	-	-	+	+	+
Lymphoid follicles^f		+2	+2	+2	0	0	+3	+1	0	+1	+3	0	+2	+1	+1	+1
Morphological changes	Surface epithelial injury	+	+	+	-	+	+	-	-	+	+	-	+	+	+	-
Morphological changes	Crypt hyperplasia	-	-	+	-	+	-	+	-	+	+	-	-	-	-	+
	Crypt dilation/distortion	+	-	+	-	+	-	+	-	+	+	-	-	-	-	-
	Mucosal fibrosis and atrophy	+	+	+	-	-	+	+	-	-	+	-	+	-	+	-
Severity of inflammatory and morphological changes^g		Mi	Mo	Ma	No	Mi	Ma	Mi	No	Mi	Ma	No	Mo	Mi	Mo	Mi

^aM = male; F = female.
^b1 ≤ one year-old age; one year-old age < 2 ≤ five year-old age.
^cA = anorexia; H = healthy; MD = mild diarrhea; W = weight loss.
^d0 = [M = 0]; +1 = [M ≤ 10]; +2 = [10 < M ≤ 50]; +3 = [M ≥ 50]; [M = Mean Helicobacter spirochetes number in 10 microscopic field (Giemsa, ×1000)].
^eLP = lymphocytes and plasma cells; N = neotrophils; E = eosinophils; Mmacrophage.
^f0 = without lymphoid follicle; +1 = presence of one lymphoid follicle; +2 = presence of two lymphoid follicles; +3 = presence of three or more than three lymphoid follicles.
^gNo = normal (healthy dog); Mi = mild (Leukocytes appearance ≤ 10, with mild morphologic changes); Mo = moderate (10 < leukocytes ≤ 50, with moderate morphological changes); Ma = marked (leukocytes ≥ 50, with marked morphological changes).

ISSN: 1746-1596