Table 2 Comparison of sensitivity and specificity among real-time PCR, conventional PCR, IHC and ISH for detecting MCPyV-infection
Target gene | Kinds of examinations | Sensitivity | Specificity |
|---|---|---|---|
MCPyV-LT | Real-time PCR | 1.0 | 1.0 |
IHC (CM2B4) | 0.94 | 0.94 | |
MCPyV-ST | Conventional PCR for ST DNA | 0.94 | 1.0 |
IHC (ST-1) | |||
Nuclear reaction | 0.69 | 1.0 | |
Cytoplasmic reaction | 0.88 | 0 | |
Real-time PCR for ST mRNA | 1.0 | ND | |
ISH for ST mRNA | 0.94 | 1.0 |