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Table 2 Comparison of sensitivity and specificity among real-time PCR, conventional PCR, IHC and ISH for detecting MCPyV-infection

From: A new in situ hybridization and immunohistochemistry with a novel antibody to detect small T-antigen expressions of Merkel cell polyomavirus (MCPyV)

Target gene Kinds of examinations Sensitivity Specificity
MCPyV-LT Real-time PCR 1.0 1.0
IHC (CM2B4) 0.94 0.94
MCPyV-ST Conventional PCR for ST DNA 0.94 1.0
IHC (ST-1)   
Nuclear reaction 0.69 1.0
Cytoplasmic reaction 0.88 0
Real-time PCR for ST mRNA 1.0 ND
  ISH for ST mRNA 0.94 1.0