Figure 2

Quantitative RT-PCR detection of APMI and Visfatin genes in preadipocytes infected with Ad36. The 3T3-L1 preadipocytes were infected with Ad36 (3.8 MOI or 7.6 MOI). The mock-infected cells were used as the negative controls. The cells were harvested at day 1, 3, 5, 7 days after infection. The total RNAs were extracted and quantitative RT-PCR was performed to determine the mRNA levels. Adenoviruses with titers of 3.8 MOI or 7.6 MOI were used to infect the cells, respectively. (A) E4orf1 expression levels in cells infected with Ad36 (3.8 MOI or 7.6 MOI); (B) APMI mRNA levels in cells infected with Ad36 of 3.8 MOI; (C) Visfatin mRNA levels in cells infected with Ad36 of 3.8 MOI; (D) APMI mRNA levels in cells infected with Ad36 of 7.6 MOI; (E) Visfatin mRNA levels in cells infected with Ad36 of 7.6 MOI.