Figure 3

ImageJ analyses of nuclear morphology. To quantify the morphological changes in cell nuclei upon staurosporine incubation, cell nuclei were counterstained with DAPI and nuclear morphology was assessed with ImageJ. Photomicrographs (16-bit) of DAPI stained nuclei of control cells (A) were converted to 8-bit images, then auto-thresholded by the “Make Binary” function, using the default method. Touching nuclei were then separated with the “Watershed” function (B). Thereafter, the “Analyze Particles” function was used to analyze nuclear morphology (C). Photomicrographs of staurosporine-incubated cells were processed identically (D-F). ImageJ excluded nuclei residing on the edge of the image through the “Exclude on Edges” function and small nuclear fragments were omitted from the measurements by adjusting the particle area range for the analysis. Original magnification: ×200. Photomicrographs are representative of three (A) and five (B) samples.