Figure 3
From: Modulation of insulin/IGFs pathways by sirtuin-7 inhibition in drug-induced chemoreistance
mRNA and protein expression of INSR in drug-resistant cell lines. A) mRNA expression of INSR normalized to GAPDH mRNA expression by RT-PCR; B) A representative Western blot for INSR protein expression showing a significant reduction of INSR expression in drug-resistant cell lines (MCF-7, SaOS2, and A2780); C) Densitometric quantitation of INSR/GAPDH protein expression ratio.