Figure 5
From: The development of endometrial hyperplasia in aged PD-1-deficient female mice
The characteristic and anatomic distribution of VEGF in uteri from 2-year old WT and PD-1-/-mice. Immunohistochemistry detection showed (A) no mouse positive staining using IgG isotype control antibodies; (B) the VEGF-positive cells were weakly observed in the glandular/luminal epithelium of WT uteri; (C) Strong VEGF-positive staining was observed in the glandular/luminal epithelium from PD-1-/- uteri. ▲ indicates the glandular epithelium; *indicates the luminal cavity and arrows showed the positive cells; (D) immunofluorescent double staining showed that VEGF is expressed on CD3+ T cells, CD56+ NK cells, CK-18+ epithelial cells, CD68+ macrophages and PD-1+ cells. The blue color indicates nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI), and arrows showed the positive cells. Scale bar = 20 μm. N = 4 of each group.