Digital immunohistochemistry platform for the staining variation monitoring based on integration of image and statistical analyses with laboratory information system

Laurinaviciene, Aida; Plancoulaine, Benoit; Baltrusaityte, Indra; Meskauskas, Raimundas; Besusparis, Justinas; Lesciute-Krilaviciene, Daiva; Raudeliunas, Darius; Iqbal, Yasir; Herlin, Paulette; Laurinavicius, Arvydas

doi:10.1186/1746-1596-9-S1-S10

Table 1 Slide-to-slide IHC staining variation of the 10 multi-tissue control samples, based on IA output variables.

From: Digital immunohistochemistry platform for the staining variation monitoring based on integration of image and statistical analyses with laboratory information system

SampleID	1	2	3	4	5	6	7	8	9	10
N	69	69	69	69	69	69	69	69	69	69
Mean
Total Nuclei	2111	1006	859	1175	458	496	928	587	3059	120
Positive Nuclei	1436	541	310	400	74	89	122	111	2436	25
Percent Positive Nuclei	68	55	36	35	19	19	14	20	79	23
Positive Density	1462	895	752	616	285	270	250	373	1810	262
Negative Nuclei	674	464	550	775	384	406	806	476	623	95
Negative Density	694	749	1320	1160	1306	1178	1615	1504	464	882
Area of Analysis	0,97	0,60	0,41	0,66	0,26	0,32	0,50	0,31	1,34	0,09
Brown Intensity	110	138	133	126	174	174	171	163	106	183
Blue Intensity	167	171	170	170	173	172	170	170	171	179
Intensity Brown/Blue Ratio	0.66	0.81	0.78	0.74	1.01	1.01	1.01	0.96	0.62	1.03
Blue Area	0.10	0.12	0.13	0.18	0.18	0.18	0.20	0.14	0.12	0.09
Brown Area	0.36	0.22	0.07	0.09	0.05	0.05	0.05	0.04	0.61	0.02
Standard deviation
Total Nuclei	527	454	275	380	423	393	487	377	524	162
Positive Nuclei	408	235	96	112	71	79	74	73	515	28
Percent Positive Nuclei	5	5	3	3	7	7	4	5	4	8
Positive Density	178	84	66	66	85	111	65	84	334	118
Negative Nuclei	164	232	183	273	357	326	422	310	106	136
Negative Density	103	148	136	102	321	244	214	233	74	290
Area of Analysis	0,22	0,24	0,13	0,21	0,22	0,22	0,27	0,19	0,07	0,10
Brown Intensity	3.61	4.83	5.85	5.32	2.51	3.03	2.27	4.61	5.80	2.98
Blue Intensity	3.21	2.86	3.50	2.75	3.27	3.99	4.08	4.35	2.53	3.51
Intensity Brown/Blue Ratio	0.02	0.03	0.04	0.04	0.02	0.02	0.03	0.04	0.03	0.02
Blue Area	0.03	0.02	0.03	0.04	0.04	0.04	0.03	0.03	0.02	0.08
Brown Area	0.12	0.09	0.02	0.02	0.02	0.02	0.02	0.01	0.10	0.02
Relative error
Total Nuclei	0.25	0.45	0.32	0.32	0.92	0.79	0.52	0.64	0.17	1.35
Positive Nuclei	0.28	0.43	0.31	0.28	0.96	0.89	0.60	0.66	0.21	1.16
Percent Positive Nuclei	0.07	0.09	0.08	0.08	0.37	0.39	0.28	0.27	0.05	0.35
Positive Density	0.12	0.09	0.09	0.11	0.30	0.41	0.26	0.23	0.18	0.45
Negative Nuclei	0.24	0.50	0.33	0.35	0.93	0.80	0.52	0.65	0.17	1.42
Negative Density	0.15	0.20	0.10	0.09	0.25	0.21	0.13	0.15	0.16	0.33
Area of Analysis	0,22	0,39	0,31	0,31	0,84	0,69	0,53	0,61	0,06	1,14
Brown Intensity	0.03	0.04	0.04	0.04	0.01	0.02	0.01	0.03	0.05	0.02
Blue Intensity	0.02	0.02	0.02	0.02	0.02	0.02	0.02	0.03	0.01	0.02
Intensity Brown/Blue Ratio	0.04	0.04	0.05	0.05	0.02	0.02	0.03	0.05	0.05	0.02
Blue Area	0.27	0.15	0.27	0.22	0.25	0.22	0.13	0.22	0.17	0.88
Brown Area	0.33	0.39	0.31	0.25	0.41	0.38	0.32	0.35	0.16	0.65

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ISSN: 1746-1596

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