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Figure 2 | Diagnostic Pathology

Figure 2

From: Digital immunohistochemistry wizard: image analysis-assisted stereology tool to produce reference data set for calibration and quality control

Figure 2

Building of dD marks: Original (initial spot image), Genie (spot image processed by the Genie software), Nuclear (spot image processed by the Nuclear software in the Genie mask), Excess RGB (computation of an excess red, green and blue image from the Nuclear resulting image), Negative and Positive (binary masks resulting from Excess RGB image thresholding), Resulting dD (test grid of frames with marks of the positive, in orange, and negative, in green, nuclear sections).

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