Figure 1

HOXB7 is the target of miR-337. A) Sequence alignment of miR-337 and its target sites in 3'UTR of HOXB7 was revealed by four prediction programs (miRBase, RNA22, miRanda, and TargetScan). B) Western blot was performed to detect the expression of HOXB7 protein in PANC-1 and AsPC-1. The levels of HOXB7 protein were lower in cells transfected with miR-337 mimics than in control cells (untransfected or transfected with NC). C) There was significantly less relative luciferase activity in cells cotransfected with HOXB7-3'UTR-WT and miR-337 mimics than in cells cotransfected with HOXB7-3'UTR-MUT and miR-337 mimics or in cells cotransfected with HOXB7-3'UTR-WT (or HOXB7-3'UTR-MUT) and NC. miR-337 mimics obviously inhibited luciferase activity in cells transfected with HOXB7-3'UTR-WT. Asterisks indicate significance at P <0.05.