Figure 2

miR-337 reduced cell proliferation in vitro. The effects of miR-337 mimics (or NC) on the proliferation of PANC-1 and As-PC-1 cells in vitro were detected by CCK-8 assay and colony formation assay. A) After transfection with miR-337 mimics (or NC), PANC-1 and As-PC-1cells were seeded in a 96-well plate and incubated for four days. CCK-8 assay shows the absorbance of cells transfected with miR-337 mimics decreased compared to the control groups (NC and blank control) on days 0-4. B) Twenty-four hours after transfection, PANC-1 or As-PC-1cells were plated on 6-well plates and cultured of 2 weeks. A colony formation assay shows that cells transfected with miR-337 mimics formed significantly fewer colonies than the control groups (NC and blank control). Asterisks indicate significance at P < 0.05.