Table 1 Primer and probe sequences for 5′-exonuclease assays for IL-22 polymorphisms
From: Association of the interleukin-22 genetic polymorphisms with ulcerative colitis
SNPs | rs2227485 | rs1182844 | rs1179246 |
|---|---|---|---|
Exchange | -429 C/T | +1046 T/A | +1995 A/C |
Forward Primer | AAAATGAGTCCGTGACCAAAATGC | CCACCTATGAGACTTCCCTATCAGT | GAAAAAGCCTTCCTGCCTAATGG |
Reverse Primer | ACACAATTGTTTTGTCTTAGTAGAGTTCAGAT | CACTAAAGGAAAAGGAAAGCTGTGTTT | GGTGCTGCCTAAAGGTCAGA |
Wildtype-Probe | FAM-CTCCTATAGTGACTGAGTAA-NFQ | VIC-AAACTTACTAGTAGGTATGACTC-NFQ | VIC-TGAACAGAGTTATCTGCCTC-NFQ |
Mutant-Probe | VIC-CTCCTATAGTGGCTGAGTAA-NFQ | FAM-CTTACTAGTAGGAATGACTC-NFQ | FAM-AACAGAGTTAGCTGCCTC-NFQ |