IGF-1R is a direct target of miR-7 in glioma cells. (A) Diagram of seed sequence of miR-7 matched the 3′UTR of the IGF-1R gene. (B) Luciferase reporter assays in glioma cells, following co-transfection of cells with wild-type or mutant 3′UTR IGF1R and miRNA, as indicated. (C) Effect of miR-7 on IGF-1R, AKT, pAKT protein levels. Each data point represents the mean ± SD of three experiments. **p <0.01.