Skip to main content

Advertisement

Table 1 cDNA Primer sequences for real-time RT-PCR

From: Valsartan blocks thrombospondin/transforming growth factor/Smads to inhibit aortic remodeling in diabetic rats

Signaling components Primers
TSP 1 Forward: 5’-GGAAGAGCATCACGCTGTTTG-3’
  Reverse: 5’-GCGCTCTCCATCTTGTCACA-3’
TGFβ1 Forward: 5’ TTGCCCTCT ACAACCAACACAA-3’
  Reverse: 5’-GGCTTGCGACCCACGTAGTA- 3’
TβRII Forward: 5’ TCA CCT ACC ACG GCT TCA CTC T 3’
  Reverse: 5’ CGC CCT TTT CTT TTC CTT CA 3’
Smad2 Forward: 5’- TGT GCA GAG CCC CAA CTG TA -3’
  Reverse: 5’- TGG TGG GAT TTT GCA CAC TGT -3’
Smad3 Forward: 5’- CAA CCC CTC AGG TTC TCTGAA G -3’
  Reverse: 5’- GCA GTC CAC AGA CCA TGT CAA -3’
β-actin Forward: 5’- TTC AAC ACC CCA GCC ATG T -3’
  Reverse: 5’- GTG GTA CGA CCA GAG GCA TAC A -3’
  1. Abbreviations: TGFβ1 transforming growth factor β1, TβRII TGF β type II receptor, TSP1 thrombospondin 1.