Relative lncRNA ANRIL expression and its clinical significance in NSCLC patients. A. Relative expression of lncRNA ANRIL in NSCLC tissues in comparison with adjacent non-tumor tissues. lncRNA ANRIL expression was examined by qRT-PCR and normalized to GAPDH expression. B. The 87 total NSCLC patients included in the study were divided into an relative high-ANRIL group (n = 48) and relative low-ANRIL group (n = 39) according to the mean value of relative lncRNA ANRIL expression. C. Kaplan-Meier overall survival curves according to lncRNA ANRIL expression level. Results are expressed as mean ± SD for three replicate determination. * P <0.05.