Relative lncRNA ANRIL expression level in lung cancer cells. A. Analysis of lncRNA ANRIL expression levels in NSCLC cell lines (A549, SPC-A1, NCI-H1650) compared with the normal bronchial epithelial cell line (16HBE) by qRT-PCR. B. Analysis of lncRNA ANRIL expression following treatment of SPC-A1 cells with si-ANRIL or si-NC by qRT-PCR. Results are expressed as mean ± SD for three replicate determination. * P <0.05.