3D reconstruction and virtual sectioning of coronaries. A and B. 3D reconstruction (xy/xz/yz planes and 3D) and graphical representation of an obliquely cut vessel with minimal CAV. The xy plane (upper left) is digital image of the tissue as it appears after initial sectioning. C. After initial 3D reconstruction, the virtual specimen was rotated and sectioned digitally into true cross sections of the coronary artery (Upon rotation the image of the virtual planes are changing, the 3D object does not). D. Graphical representation of the virtual rotation and resampling. E. 3D reconstruction of a curved and obliquely cut coronary artery with severe CAV. In this situation, conventional histology is unlikely to obtain precise measurements of the NI. F. Graphical representation of a section plane with a falsely elevated NI, and a rotated virtual section plane with an accurate NI.