Neointimal volume index, virtual coronary angiography. Extracted lumen (A) and neointima (B) of a distal coronary portion from sample A. C/D: Through image modulation, color filtration and digital subtraction, the vasculature is highlighted and the background tissue suppressed, generating a virtual coronary angiography. (yellow circle: branching coronary – LAD, yellow star: non branching coronary – RCA.) E. Fluorescent scanning of an HE stained slide. The internal and external elastic lamina of the coronary artery are bright in this false color image, as are structures in the cardiac parenchyma with high collagen content. F. 3D reconstruction of the vasculature from HE stained slides imaged using fluorescence scanning.