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Table 2 Comparison of flow cytometric features among normal, non-clonal cytopenias and MDS

From: Improving the differential diagnosis between myelodysplastic syndromes and reactive peripheral cytopenias by multiparametric flow cytometry: the role of B-cell precursors

  Normal values n = 41 Reactive cytopenias n = 35 MDS <5% BM blasts n = 42 MDS >5% BM blasts n = 12 p
Granulocytic series      
SSC 521 (396–658) 534 (340–628) 394 (236–619) 462 (319–648) <0.0001
CD45 MFI 138-577 265 (107–683) 216 (34–770) 270 (89–551) 0.02
Asynchronous shift to the left   7 (21%) 11 (26%) 6 (46%) 0.12
Monocytic series      
SSC 253 (196–399) 237 (149–390) 233 (66–399) 203 (184–322) 0.01
% monocytes 0.8-3.6 3.0 (0.7 - 8.8) 3.7 (0.2 - 18) 3.3 (0.1 - 21) 0.22
% CD16+ monocytes <0.48 0.3 (0.1 - 3.4) 0.4 (0.02 - 10) 0.9 (0.05 - 4) 0.003
Cells in the blast gate      
BM % blasts (cytology)   1.0 (0.5 - 4.0) 1.0 (0.5 - 5) 12 (7.7 - 18)  
% CD34+ cells <1.59 0.6 (0.02-2.1) 1.0 (0.02- 5.1) 3.6 (0.2 - 28) <0.0001
% B cell precursors/total cells >0.05 (0.04-0.53) 0.06 (0–0.7) 0.06 (0–0.7) 0.01 (0.01 - 0.14) <0.0001
%CD34+/CD13+/CD117+ <0.62 0.3 (0.01-0.8) 0.5 (0.01-4.2) 1.4 (0–24.1) 0.001
% CD34+/CD7+ <0.08 0.05 (0–0.09) 0.04 (0–0.7) 0.13 (0.04-26) <0.0001
% CD34+/CD56+ <0.05 0.01 (0–0.09) 0.03 (0–1.9) 0.13 (0.02-27) <0.0001
Total abnormalities 0 (0–2) 2 (0–5) 7 (3–13) 9 (6–16) <0.0001