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Table 3 Dilution experiment LNA assay for EGFR exon 19 deletions

From: Validation of a locked nucleic acid based wild-type blocking PCR for the detection of EGFR exon 18/19 mutations

Sample

Cp controle

Cp LNA

∆Cp

100 % mutant

29.66

30.93

1.27

50 % mutant

29.89

31.91

2.02

25 % mutant

30.13

33.37

3.24

12.5 % mutant

30.12

33.92

3.80

6.25 % mutant

30.35

35.78

5.43

3.125 % mutant

30.56

36.34

5.78

1.5625 % mutant

30.21

36.96

6.75

100 % wild-type

29.93

39.70

9.77

  1. Cp: crossing point; LNA: locked nucleic acid
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Diagnostic Pathology

ISSN: 1746-1596

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