Table 2 Score for each histological finding for the generation of histological grade for the DCIS classification systems: Holland, Van Nuys and modified Black nuclear grade system

G1 if final score <6

G1 if final score <50

G1 if final score <6

(1) monomorphic nuclei of uniform size, regular outline and spacing

(10) Nuclei 1.5 -2 RBC diameter or nuclear size in relation to normal duct: 2 fold variation in nuclear diameter

(1) monomorphic nuclei of uniform size, regular outline and spacing

or

(1) uniform, fine chromatin

(1) Nuclei 1-1.5 RBC diameter or nuclear size in relation to normal duct: similar, minimal enlargement

(1) Nuclei 1-1.5 RBC diameter or nuclear size in relation to normal duct: similar, minimal enlargement

(1) no nucleoli

(10) fine to coarse chromatin

(1) uniform, fine chromatin

or

(1) no mitoses

(1) uniform, fine chromatin

(1) no nucleoli

(1) all the cells have a well-defined apex and show polarization

(2) nucleoli insignificant

(1) no mitoses

or

(1) no nucleoli

(10) any confluent necrosis

or

(1) no necrosis

G2 if final score >5 and <90

G2 if final score >1000 and <1030

G2 if final score >5 and <90 or >13 and <16 if no mitoses

(10) nuclei showing some variation in nuclear size, outline and spacing

(10) Nuclei 1.5 -2 RBC diameter or nuclear size in relation to normal duct: 2 fold variation in nuclear diameter

(10) nuclei showing some variation in nuclear size, outline and spacing

or

or

or

(1) monomorphic nuclei of uniform size, regular outline and spacing

(1) Nuclei 1-1.5 RBC diameter or nuclear size in relation to normal duct: similar, minimal enlargement

(1) monomorphic nuclei of uniform size, regular outline and spacing

(10) fine to coarse chromatin

(10) fine to coarse chromatin

(10) nuclei 1.5 -2 RBC diameter or nuclear size in relation to normal duct: 2 fold variation in nuclear diameter

or

or

or

(1) uniform, fine chromatin

(1) uniform, fine chromatin

(1) nuclei 1-1.5 RBC diameter or nuclear size in relation to normal duct: similar, minimal enlargement

(2) nucleoli insignificant)

(2) nucleoli insignificant

(10) fine to coarse chromatin

or

or

or

(1) no nucleoli

(1) no nucleoli

(1) uniform, fine chromatin

(10) mitoses present

(1000) proeminent central necrosis - comedonecrosis

(2) nucleoli insignificant

or

or

(1) absent

(1) no nucleoli

(10) polarization present but not pronouced

(1) no mitoses

or

(1) all the cells have a well-defined apex and show polarization

G3 if final score >95

G3 if final score >100 and <320 or >1100

G3 if final score >95 or >13 and <16 if mitoses are present

(100) pleomorphic nuclei, anisonucleosis, usually but not always large

(100) nuclei >2 RBC diameter or nuclear size in relation to normal duc: 3 fold variation in nuclear diameter

(100) pleomorphic nuclei, anisonucleosis, usually but not always large

or

or

or

(10) nuclei showing some variation in nuclear size, outline and spacing

(10) nuclei 1.5 -2 RBC diameter or nuclear size in relation to normal duct: 2 fold variation in nuclear diameter

(10) nuclei showing some variation in nuclear size, outline and spacing

or

or

or

(1) monomorphic nuclei of uniform size, regular outline and spacing

(1) nuclei 1-1.5 RBC diameter or nuclear size in relation to normal duct: similar, minimal enlargement

(1) monomorphic nuclei of uniform size, regular outline and spacing

(100) coarse, clumped chromatin

(100) coarse, clumped chromatin

(100) nuclei >2 RBC diameter or nuclear size in relation to normal duc: 3 fold variation in nuclear diameter

or

or

or

(10) fine to coarse chromatin

(10) fine to coarse chromatin

(10) nuclei 1.5 -2 RBC diameter or nuclear size in relation to normal duct: 2 fold variation in nuclear diameter

or

or

or

(1) uniform, fine chromatin

(1) uniform, fine chromatin

(1) nuclei 1-1.5 RBC diameter or nuclear size in relation to normal duct: similar, minimal enlargement

(10) evident nucleoli

(10) evident nucleoli

(100) coarse, clumped chromatin

or

or

or

(2) nucleoli insignificant

(2) nucleoli insignificant

(10) fine to coarse chromatin

or

or

or

(1) no nucleoli

(1) no nucleoli

(1) uniform, fine chromatin

(10) mitoses present

(1000) proeminent central necrosis - comedonecrosis

(10) evident nucleoli

or

or

or

(1) mitoses absent

(10) any confluent necrosis

(2) nucleoli insignificant

or

or

(100) no true polarization of the cells with orientation toward a lumen

(1) no necrosis

(1) no nucleoli

or

(10) polarization present but not pronouced

(10) present mitoses

or

or

(1) all the cells have a well-defined apex and show polarization

(1) absent