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Fig. 4 | Diagnostic Pathology

Fig. 4

From: RETRACTED ARTICLE: The microRNA-325 inhibits hepatocellular carcinoma progression by targeting high mobility group box 1

Fig. 4

HMGB1 was the direct targets of miR-325. a The potential miR-325 seed region at the 3'-UTR of HMGB1 mRNA was predicted by using multiple bioinformatics analyses. HepG2 cells were co-transfected with miR-325 mimics (or NC) with pGL3-HMGB1 (or pGL3-HMGB1-mut) vector. Luciferase activity was normalized by the ratio of firefly and Renilla luciferase signals. b HMGB1 mRNA and protein expression levels in HepG2 (left) and Huh7 (right) cells transfected with NC, miR-325 mimics, inhibitor NC and miR-325 inhibitor were detected by using qRT-PCR and western-blotting. GAPDH was regarded as an internal control. Data are shown as mean ± SEM of three independent experiments. * indicates significant difference compared with control group (P < 0.05)

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