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Fig. 5 | Diagnostic Pathology

Fig. 5

From: RETRACTED ARTICLE: The microRNA-325 inhibits hepatocellular carcinoma progression by targeting high mobility group box 1

Fig. 5

Down-regulation of HMGB1 by siRNA partially reverses the promotion of cell invasion and proliferation induced by miR-325 inhibition. a The transfection efficiency was validated by using western-blotting for both HepG2 and Huh7 cells transfected with miR-325 inhibitor and inhibitor + siRNA/HMGB1 (the co-transfection cells of miR-325 inhibitor and siRNA/HMGB1) and inhibitor + siRNA/control (co-transfected with miR-325 inhibitor and siRNA/control). GAPDH was used as a control. Averages of three independent experiments were shown in the histogram (right panel). b Absorbance at 490 nm was presented with Mean ± SEM. HepG2 (left) and Huh7 (right). c Transwell assay was performed as described in Material and Methods. Cells were treated with miR-325 inhibitor and inhibitor + siRNA/HMGB1 (the co-transfection cells of miR-325 inhibitor and siRNA/HMGB1) and inhibitor + siRNA/control (co-transfected with miR-325 inhibitor and siRNA/control) for 24 h. The representative images of invasive cells stained with crystal violet were shown at the bottom. * indicates P < 0.05

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