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Fig. 1 | Diagnostic Pathology

Fig. 1

From: RETRACTED ARTICLE: HMGB1: a novel protein that induced platelets active and aggregation via Toll-like receptor-4, NF-κB and cGMP dependent mechanisms

Fig. 1

Platelets express HMGB1. a Representative immunoblot of HMGB1 expressions in WPs (1 × 109/ml) from five different health donors, Western blot analysis of extracts HMGB1 protein by HMGB1 polyclonal antibodies. b HMGB1 was assessed by ELISA in pellets and supernatant of WPs that either resting or adding thrombin (0.2 U/ml). After WPs were activated, the concentration of HMGB1 in pellets or in the supernatant signicantly different from the resting WPs group (n = 5, *P < 0.01). c WPs incubated with or without (negative controls) rabbit polyclonal-anti-HMGB1 (blue) in combination with rabbit-anti-Actin (red), respectively. Images under fluorescent microscopy, the results indicated that the resting WPs high expression of HMGB1 protein

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