Fig. 3

HMGB1 enhanced agonists-induced platelet aggregation. a A subthreshold concentration of thrombin (0.2 U/ml) or collagen (0.3 μg/ml) was added to WPs which were preincubeted with HMGB1 (1 μg/ml) or ALB for 10 min at 37 °C, above is representive platelet aggregation curve. b Data are expressed as mean ± SE, HMGB1 (1 μg/ml) that significantly enhancing thrombin or collagen-induced platelet aggregation rate (n = 5, *P < 0.05. vs collagen + ALB or thrombin + ALB group respectively). c Representative map of thromboelastogram (TEG) analyzes, the whole blood prior stimulated with HMGB1 (1 μg/ml) for 10 min at 37 °C, this one Maximum Amplitude (MA) was 77.1 mm. d Analyzing quantitative data from C, the MA of the HMGB1 group were enhanced (n = 5, *P < 0.05. vs HMGB1 + ALB)