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Fig. 4 | Diagnostic Pathology

Fig. 4

From: RETRACTED ARTICLE: HMGB1: a novel protein that induced platelets active and aggregation via Toll-like receptor-4, NF-κB and cGMP dependent mechanisms

Fig. 4

a ATP release was determined (by ATP Assay Kit) in rHMGB1-incubated WPs (1 × 109/ml), WPs were treated with rHMGB1 (1-80 μg/ml) in an aggregometer (1200 rpm) for 5 min at 37 °C (n = 6, *P < 0.05 vs. rest group). b WPs were pre-incubated with various concentrations of HMGB1 protein for 10 min, fixed with paraformaldehyde, then incubated with HMGB1 in the presence of a monoclonal anti-human P-selectin Ab or control mouse IgG (Rest) for 30 min at room temperature. (n = 6, *P > 0.05; **P < 0.05, vs. rest group). c Flow cytometric determination of P-selectin expression level. d The effects of LPS and PMB on rHMGB1-induced ATP in WPs. (n = 6, *P > 0.05; **P < 0.05, vs. rest group)

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