Fig. 6

HMGB-induced platelet activation dependent on TLR4, the data shown here are representatives of at five experiments from different donors. Results are Means ± SD. Prior to stimulation with HMGB1 (40 μg/ml), WPs were pretreatmented with various conceration of anti-TLR4 Abs, anti-TLR2 Abs, anti-RAGE Abs, or irrelevant control concerationg IgG2a in an aggregometer (1200 rpm) for 20 min respectively. Representative aggregation curves with anti-TLR4 (a), anti-TLR2 (b), anti-RAGE (c) or IgG2a (d) are shown. e, f Statistical analysis of the maximum platelet aggregation rata (n = 5, *P < 0.05, **P < 0.01, vs IgG2a) and P-selectin expression data (n = 5, *P < 0.05 vs IgG2a). g Representative flow cytometric data showed that reduced HMGB1-induced P-selectin expression