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Fig. 1 | Diagnostic Pathology

Fig. 1

From: NRAS Q61R, BRAF V600E immunohistochemistry: a concomitant tool for mutation screening in melanomas

Fig. 1

Examples of paired immunohistochemistry and pyrosequencing results. a, b, c, d case #15: primary BRAF V600 wild-type (a) and NRAS Q61R mutated (b) melanoma with strong 4+ immunostaining with SP 174 anti-NRASQ61R antibody using Red (c) or DAB (d) revelation. e, f, g, h case #18: primary melanoma with BRAF V600 (e) and NRAS Q61 wild-type (f) molecular status but presenting strong 4+ staining using VE1 anti-BRAFV600E antibody (g) and no staining with SP 174 anti-NRASQ61R antibody (h). i, j, k, l case #61: primary BRAF V600 wild-type (i) and NRAS Q61K mutated (j) melanoma having moderate staining using VE1 anti-BRAFV600E antibody (k) and fainter staining with SP 174 anti-NRASQ61R antibody (l). We concluded in a non-specific ambiguous staining in this sample with both antibodies. Note that red revelation kit as been used here as the faint melanin-pigmentation could simulate a weak DAB staining. m, n, o, p case #39: BRAF V600 (m) and NRAS Q61 wild-type (n) melanoma mesentery metastasis presenting both strong melanin pigment that could simulate a strong DAB staining and red immunostained cells with both VE1 anti-BRAFV600E (o) and SP 174 anti-NRASQ61R (p) antibodies. We retrospectively concluded that all stained cells were macrophages, without any evidence of viable tumor cells in this pigmented sample. q, r case #55: BRAF V600E mutated (q) melanoma skin metastasis with strong 4+ immunostaining with VE1 anti-BRAFV600E antibody (r)

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