Table 2 Pyrosequencing primers and parameters for genotyping the codons 600-BRAF and 61-NRAS
From: NRAS Q61R, BRAF V600E immunohistochemistry: a concomitant tool for mutation screening in melanomas
Gene | PCR primers sequence (Forward and Reverse 5′ → 3′) | Pyrosequencing primer | Nucleotides dispensation order |
|---|---|---|---|
BRAF | Biotin-GCTTGCTCTGATAGGAAAATG | GATGGGACCCACTCCATCGAGA | GTCTACTGT |
CCACAAAATGGATCCAGACA | |||
NRAS | ACACCCCCAGGATTCTTACAGA | GACATACTGGATACAGCTGGA | TCGTATCGAGAG |
Biotin-GCCTGTCCTCATGTATTGGTC |