Fig. 5

In situ hybridization for CA7 and CXCL1 mRNAs. Paraffin sections from normal colon (a-d), colon adenoma (e-h) and colon cancer (i-l) were stained with LNA probes for CXCL1 mRNA (a,e,i), CA7 mRNA (b,f,j), a generic unspecific sequence, scramble (c,g,k), and a positive control probe, miR-126 (d,h,l). The CXCL1 ISH signal is seen in a population of macrophage-like cells located in the cancer stroma (i, arrows) just below the luminal ulceration (indicated by U), whereas no CXCL7 ISH signal is detected in the normal colon mucosa and in the adenoma (a,e). The CA7 ISH signal is seen in a subset of epithelial cells in normal colon mucosa (b, arrows), whereas no ISH signal is detected in the colon adenoma and cancer tissue (f,j). miR-126 ISH signal is prevalent in endothelial cells (arrows in d,h,l), and only background staining is seen with scramble probe (c,g,k). Tissue sections were counter stained with Nuclear Fast Red. 50 μm bar is representative for all images