Skip to main content
Fig. 3 | Diagnostic Pathology

Fig. 3

From: Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique

Fig. 3

Representative examples of MALDI MS results. On the left, 16 cases plus wild type controle and no-template control tested for a KRAS mutation (p.Q61H). 15 cases are KRAS wild-type (including the wild-type control); one of the cases is highlighted with an arrow (a). In this specific case the sequencing primer (first arrow) is completely elongated by an Adenin indicated by a high peak in the mass range of the wild-type allele (second arrow) (b). One case shows a mutation in KRAS (c.183A > C, p.Q61H) (c). The reverse sequence-specific primer is completely consumed and elongated by either Thymine or Guanine (wild-type and mutated allele; arrows) (d)

Back to article page

Diagnostic Pathology

ISSN: 1746-1596

Contact us