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Table 3 Estimated Hands-on-time and Time-to-results

From: Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique

KRAS LCD-array Kit

Hands-on-time in minutes

Time-to-result in minutes

PCR reaction

15

150

Hybridization

20

60

Evaluation on PC

10

10

Overall

45

220

Sanger sequencing

  

ᅟPCR reaction

15

150

ᅟGel preparation and electrophoresis

25

45

ᅟDucumentation and Evaluation of Dilution

10

10

ᅟEthanolprecipitation and centrifugation

20

35

ᅟElution of the Pellets in pure water

10

10

ᅟSequencing reaction

10

160

ᅟAdd purification beads and Incubation

10

40

ᅟSequencing run for all exons (forward and reverse)

10

250

ᅟEvaluation on PC

10

15

ᅟOverall

120

715

ᅟMALDI MS

  

ᅟPCR reaction

15

150

ᅟDephosphorylation SAP reaction

5

60

ᅟCycled MassEXTEND reaction

10

150

ᅟSample conditioning, Nanodispensing

10

40

ᅟMS analysis and calling

5

30

ᅟOverall

45

430

ᅟNGS

  

ᅟLibrary Preparation

30

300

ᅟEmulsion PCR

10

360

ᅟSequencing

20

300

ᅟData analysis

10

60

ᅟOverall

70

1020