KRAS LCD-array Kit | Hands-on-time in minutes | Time-to-result in minutes |
---|---|---|
PCR reaction | 15 | 150 |
Hybridization | 20 | 60 |
Evaluation on PC | 10 | 10 |
Overall | 45 | 220 |
Sanger sequencing | ||
ᅟPCR reaction | 15 | 150 |
ᅟGel preparation and electrophoresis | 25 | 45 |
ᅟDucumentation and Evaluation of Dilution | 10 | 10 |
ᅟEthanolprecipitation and centrifugation | 20 | 35 |
ᅟElution of the Pellets in pure water | 10 | 10 |
ᅟSequencing reaction | 10 | 160 |
ᅟAdd purification beads and Incubation | 10 | 40 |
ᅟSequencing run for all exons (forward and reverse) | 10 | 250 |
ᅟEvaluation on PC | 10 | 15 |
ᅟOverall | 120 | 715 |
ᅟMALDI MS | ||
ᅟPCR reaction | 15 | 150 |
ᅟDephosphorylation SAP reaction | 5 | 60 |
ᅟCycled MassEXTEND reaction | 10 | 150 |
ᅟSample conditioning, Nanodispensing | 10 | 40 |
ᅟMS analysis and calling | 5 | 30 |
ᅟOverall | 45 | 430 |
ᅟNGS | ||
ᅟLibrary Preparation | 30 | 300 |
ᅟEmulsion PCR | 10 | 360 |
ᅟSequencing | 20 | 300 |
ᅟData analysis | 10 | 60 |
ᅟOverall | 70 | 1020 |