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Table 3 Estimated Hands-on-time and Time-to-results

From: Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique

KRAS LCD-array Kit Hands-on-time in minutes Time-to-result in minutes
PCR reaction 15 150
Hybridization 20 60
Evaluation on PC 10 10
Overall 45 220
Sanger sequencing   
ᅟPCR reaction 15 150
ᅟGel preparation and electrophoresis 25 45
ᅟDucumentation and Evaluation of Dilution 10 10
ᅟEthanolprecipitation and centrifugation 20 35
ᅟElution of the Pellets in pure water 10 10
ᅟSequencing reaction 10 160
ᅟAdd purification beads and Incubation 10 40
ᅟSequencing run for all exons (forward and reverse) 10 250
ᅟEvaluation on PC 10 15
ᅟOverall 120 715
ᅟMALDI MS   
ᅟPCR reaction 15 150
ᅟDephosphorylation SAP reaction 5 60
ᅟCycled MassEXTEND reaction 10 150
ᅟSample conditioning, Nanodispensing 10 40
ᅟMS analysis and calling 5 30
ᅟOverall 45 430
ᅟNGS   
ᅟLibrary Preparation 30 300
ᅟEmulsion PCR 10 360
ᅟSequencing 20 300
ᅟData analysis 10 60
ᅟOverall 70 1020