Fig. 1

The miR-1246 is often upregulated in NSCLC and promotes NSCLC cell migration and invasion. a The relative expression of mature miR-1246 in 50 pairs of NSCLC tissues and their corresponding noncancerous lung tissues were measured using TaqMan real-time PCR and normalized to U6 snRNA. b CCK-8 assays of A549 and H226 cells were performed every other day after transfection with a miR-1246 mimic or negative control (nc). The data are presented as the mean ± S.E.M. c, d Transwell migration and invasion assays of A549 and H226 cells stably expressing miR-1246 or mock control. e, f Transwell migration and invasion assays for A549 and H226 cells transfected with either miR-1246 inhibitor or negative control. Representative images are shown on the left, and quantification is shown on the right. The results are representative of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001