Fig. 4

CPEB4 suppresses miR-1246-induced migration/invasion and its expression is inversely correlated with miR-1246 in NSCLC. a, b Real-time PCR and western blot of CPEB4 expression in HEK 293 T cells transfected with siRNAs targeting CPEB4 or a negative control (nc). c Transwell migration and invasion assays of A549 cells were performed after transfection with a miR-1246 inhibitor, CPEB4 siRNA or a negative control (nc). The protein level of CPEB4 was detected through western blot. There is statistical significance between group 1 and group 2 as well as between group 2 and group 4. The results are presented as the mean ± SD. d The relative expression of CPEB4 at the mRNA level was analyzed through real-time PCR (normalized to β-actin). e The correlation between CPEB4 expression and mature miR-1246 in various lung cancer cell lines was analyzed by linear regression. f The mRNA level of CPEB4 was determined in 50 pairs of NSCLC tissues and matched noncancerous adjacent lung tissues by real-time PCR. g The correlation between CPEB4 expression and mature miR-1246 was analyzed in the same NSCLC samples by linear regression. The expression data were normalized to β-actin and U6 snRNA, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001