Fig. 1From: Rapid detection of immunoglobulin heavy chain gene rearrangement by PCR and melting curve analysis using combined FR2 and FR3 primersAnnealing temperature gradient PCR on Ramos cell line. FR2 (left) and FR3 (right) primers were used to amplify Ramos cell line DNA with gradient annealing temperature from 58 °C to 68 °C to explore the optimal annealing temperature for both multiplex-PCR setsBack to article page