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Fig. 3 | Diagnostic Pathology

Fig. 3

From: Rapid detection of immunoglobulin heavy chain gene rearrangement by PCR and melting curve analysis using combined FR2 and FR3 primers

Fig. 3

Comparison of PAGE, MCA and CE. a MCA of 5 lymphoma samples with FR2 primers. Sample 23 was negative, 24–27 were positive. b PAGE of the same 5 lymphoma samples. A very faint band can hardly be seen in sample 25# and 26# (in box) that is interpreted as undetermined and need to be further confirmed. c Evaluation of DNA quality. All samples including 25# and 26# have a sharp peak at identical Tm, which indicated the presence of lymphoid tissue with integrated DNA. d CE of the sample 25# and 26#. Both samples had a single peak at expected sizes

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