Fig. 4From: Rapid detection of immunoglobulin heavy chain gene rearrangement by PCR and melting curve analysis using combined FR2 and FR3 primersMinimal detection of MCA. Ramos cell line DNA was serially diluted with negative control at various percentages (50 %, 25 %, 12.5 %, 6.25 %, 3.125 %, 1.56 % and 0.78 %). After PCR, a clonal peak at 3.125 % can still been detected using both FR2 and FR3 primer setsBack to article page