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Table 2 MCA of two different clonal DNA samples using FR2 primers

From: Rapid detection of immunoglobulin heavy chain gene rearrangement by PCR and melting curve analysis using combined FR2 and FR3 primers

Experiment NO.

Original

After mixing

Tm1

Tm2

Tm

Tml

Tm2

1

84.45

85.18

0.73

84.45

2

84.45

85.50

1.05

84.62

3

84.45

88.48

2.03

84.41

86.56

4

84.45

87.35

2.90

84.11

86.99

5

84.45

87.68

3.23

84.23

87.50

  1. Tm: Difference of melting temperatures of two original samples individually detected by MCA