Fig. 2From: MiR-186 suppresses the growth and metastasis of bladder cancer by targeting NSBP1miR-186 directly targeted NSBP1. a, Sequence alignment of miR-186 and 3′ UTR of NSBP1 using mirco-RNA.org. Luciferase reporter assay with co-transfection of wild-type or mutant NSBP1 and miR-186 mimics or miR–control (scramble control mimics).in HEK 293 T cells. Error bars represent ± S.E. and *, p < 0.01 versus negative control (scramble control mimics). b, qRT-PCR analysis revealed the effects of NSBP1 siRNA and miR-186 mimics on the expression level of miR-186. c, Western blot analysis revealed the effects of NSBP1 siRNA and miR-186 mimics on the expression level of NSBP1. Error bars represent ± S.E. and *, p < 0.01 versus NC (scramble control siRNA)Back to article page