Table 1 Restriction enzyme and primer sequences of IL-8 SNPs
From: Association between interleukin 8–251 T/A and +781 C/T polymorphisms and glioma risk
Gene and SNP | Enzyme | Forward primer | Reverse primer | Product |
|---|---|---|---|---|
IL-8 -251 A/T | MfeI | 5′-TCATCCATGATCTTGTTCTAA-3′ | 5′-GGAAAACGCTGTAGGTCAGA-3′ | T/T: 542 bp; A/A: 450 bp, 92 bp |
IL-8 + 781 C/T | EcoRI | 5′-CTCTAACTCTTTATATAGGAATT-3′ | 5′-GATTGATTTTATCAACAGGCA-3′ | T/T: 203 bp; C/C: 184 bp, 19 bp |