Fig. 4

Direct spatial interaction in a case with one focal, dense infiltration. Serial sections of a trephine biopsy from a patient with a follicular lymphoma were stained for CD20 and Bcl2. Slides were fully digitalized, registered and analysed as described in Methods.A-B: Staining for CD20 and Bcl2. After segmentation of nuclei, there were 352 CD20+ and 859 CD20−/+ nuclei in a, and 233 Bcl2+ and 795 Bcl2−/+ nuclei in b. c Scatter plot for all nuclei (CD20−/+ and Bcl2−/+) with PCC = 0.74, MOC = 0.97 and M_CD20−/+ = 0.90 and M_Bcl2−/+ = 0.95. d Scatter plot for the bone areas with PCC = 0.94, MOC = 0.98 and M_BoneCD20 = 0.76 and M_BoneBcl2 = 0.99. e Scatter plot for all positive nuclei (CD20+ and Bcl2+) with PCC = 0.90, MOC = 0.97 and M_Bcl2+ = 0.99 and M_CD20+ = 0.71, respectively. f-g Heat map of the diversity for CD20 (F) and Bcl2 (G)