Fig. 5

Direct spatial interaction in a lymphoid follicle. Serial sections of a trephine biopsy from a patient with bone marrow lymphocytosis were stained for CD3, CD3, CD20 and CD20 (a-d). Subsequently, slides were fully digitalized, registered and analysed as described. By segmentation, there are 320 CD3+ and 1,156 CD3−/+ nuclei in a, 392 CD3+ and 1,139 CD3−/+ nuclei in b, 316 CD20+ and 1,176 CD20−/+ nuclei in c and respectively 202 CD20+ and 1,182 CD20−/+ nuclei in d. Overlap readings are calculated for direct interaction (RBF_direct). A + B: Overlap of both images with A (first section stained for CD3) drawn in red and respectively B (second section stained for CD3) drawn in blue. For stained cells, PCC = 0.95, MOC = 0.99 and M_{1st section CD3+} = 0.98 and M_{2nd section CD3} = 0.81. B + C: Overlap of both images with B (second section stained for CD3) drawn in red and respectively C (first section stained for CD20) drawn in blue. For stained cells, PCC = 0.97, MOC = 0.99 and M_{2ndsection CD3+} = 0.83 and M_{1stsection CD20} = 0.97. C + D: Overlap of both images with C (first section stained for CD20) drawn in red and respectively D (second section stained for CD20) drawn in blue. For stained cells, PCC = 0.67, MOC = 0.92 and M_{1st section CD20+} = 0.57 and M_{2nd section CD20} = 0.97