Fig. 3

Multiplex ASPCR results. Novel variants found in the SMAD4 and RET genes were confirmed by ASPCR and capillary electrophoresis using Lab-on-a-Chip technology performed on the 4 cell lines used in this study. For the SMAD4 gene, the 286 bp peak corresponds to a conserved region encompassing the C > T variant at the 48,586,344 position in the hg19 reference genome and the 181 bp peak corresponds to the variant-specific amplicon. For the RET gene, the 268 bp peak corresponds to a conserved region encompassing the A > G variant at the 43,615,612 position in the hg19 reference genome, and the 176 bp peak corresponds to the variant-specific amplicon