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Table 1 ASPCR Primers

From: Quality control material for the detection of somatic mutations in fixed clinical specimens by next-generation sequencing

Amplicon Name

Primer Sequence (5'-3')

Amplicon Size (bp)

BRAF_conserved

BRAF-F1: TGCTTGCTCTGATAGGA

241

BRAF_R: CTAGTAACTCAGCAGCA

BRAF_V600E

BRAF-F2: TGGTCTAGCTACAGA

141

BRAF_R: CTAGTAACTCAGCAGCA

SMAD4_conserved

SMAD4_F1: TTGTCTTTTCTTTAGGGC

286

SMAD4_R: AAGATAGTTCTTTTCTTTTGG

SMAD4_48586344_C > T

SMD4_F2: ATTTAGTGGTGATTGAAAT

181

SMAD4_R: AAGATAGTTCTTTTCTTTTGG

RET_conserved

RET_F1: GTGCTATTTTTCCTCACA

268

RET_R: AGGGCTATAAAAAGCTTAG

RET_43615612_A > G

RET_F2: GCTTGTCCCGG

176

RET_R: AGGGCTATAAAAAGCTTAG